Fast and High-Accuracy Localization for Three-Dimensional Single-Particle Tracking

Fast and High-Accuracy Localization for Three-Dimensional Single-Particle Tracking

We report a non-iterative localization algorithm that utilizes the scaling of a three-dimensional (3D) image in the axial direction and focuses on evaluating the radial symmetry center of the scaled image to achieve the desired single-particle localization. Using this approach, we analyzed simulated 3D particle images by wide-field microscopy and confocal microscopy respectively, and the 3D tra...

Single-camera, three-dimensional particle tracking velocimetry.

This paper introduces single-camera, three-dimensional particle tracking velocimetry (SC3D-PTV), an image-based, single-camera technique for measuring 3-component, volumetric velocity fields in environments with limited optical access, in particular, optically accessible internal combustion engines. The optical components used for SC3D-PTV are similar to those used for two-camera stereoscopic-µ...

Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking.

Tracking of single particles in optical microscopy has been employed in studies ranging from material sciences to biophysics down to the level of single molecules. The technique intrinsically circumvents ensemble averaging and may therefore reveal directly mechanistic details of the involved dynamic processes. Such processes range from translational and rotational motion to spectral dynamics. W...

High-accuracy global localization filter for three-dimensional environments

Real-time nanomicroscopy via three-dimensional single-particle tracking.

We developed a new method for real-time, three-dimensional tracking of fluorescent particles. The instrument is based on a laser-scanning confocal microscope where the focus of the laser beam is scanned or orbited around the particle. Two confocal pinholes are used to simultaneously monitor regions immediately above and below the particle and a feedback loop is used to keep the orbit centered o...